IST Innuscreen‘s ready-to-use nucleotide mix provides highest quality of deoxynucleotides. All dNTPs are ultra pure (> 98 %) and quality checked by a set of PCR, RT-PCR and Klenow reactions. It is supplied as a 50-fold concentrated mix of ultra pure dATP, dCTP, dGTP and dTTP with 12.5 mM each. The total amount of dNTP in each tube is 25 µmol (6.25 µmol of each dNTP).
inNucleotide Set is a Set of dATP, dCTP, dGTP and dTTP in a concentration of 100 mM each. The solution is ready to use and the total amount of dNTP in each tube is 25 µmol.
The innuDRY qPCR MasterMix Probe is a novel, storage-stable real-time PCR Master Mix for fluorescence detection using different probe technologies (e.g. hydrolysis, FRET or rehybridization probes, and many more). The mix contains all components for a successful real-time PCR. Besides high-quality dNTP’s and a perfect combination of qPCR enhancers, the hot start polymerase avoids unspecific side products selectively. Additionally, the lyophilized 2-times formulation offers high storage stability at +4 °C and an environmentally friendly delivery at room temperature. After dissolving by using the Resuspension Buffer only the primer, probes and template need to be added to the master mix and filled up to the final volume by using molecular grade water. The established handling makes the daily use easy and secure. qPCR is highly reproducible and ideal in terms of efficiency and slope.
The innuDRY qRT-PCR Mix Probe is designed for efficient cDNA synthesis and subsequent qPCR in a single tube. It can be used to quantify any RNA template including mRNA, total RNA and viral sequences. Extremely low copy number targets can be detected specifically with high efficiency
In the daily routine, simple and safe processes are particularly important, which guarantee the high reproducibility of the final results. In addition, it is important that reagents used are stable and work reliably. The innuDRY Standard PCR MasterMix combines all requirements. The lyophilized mix is long-term storage stable and can be dissolved by the included Resuspension Buffer at any time. After addition of template and specific primers the PCR reaction just needs to be filled up to final volume by using molecular grade water and is ready-to-use for subsequent amplification. The use of innuDRY Standard PCR MasterMix can be easily combined with most commercially available thermal cyclers. Thereby quality as well as yield of amplified PCR products is excellent.
The innuMIX qPCR DSGreen Standard is one of the best choice products for fast and highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments with NO-ROX requirements. It contains all reagents required for real-time PCR and is designed to achieve excellent results in reaction efficiency and slope.
The innuMIX qPCR MasterMix Probe has been developed for fast, highly reproducible, real-time PCR and validated on the most common real-time instruments. The master mix can be readily combined with a wide variety of probe systems, including TaqMan and rehybridization probes.
The innuMIX qPCR MasterMix SyGreen Sensitive was developed for fast, highly reproducible real-time PCR and validated on the most popular real-time PCR devices for low-ROX and no-ROX. The innuMIX qPCR MasterMix SyGreen uses a proprietary intercalating dye, which does not inhibit PCR. The MasterMix can be used to quantify any DNA template including genomic, cDNA and viral sequences. The proprietary technology prevents formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity.
The innuPREP Bacteria Lysis Booster has been developed for a highly efficient non-mechanical pre-lysis of bacterial cell walls by generating spheroblasts. This new mixture of different enzymes boosts the lysis of all bacteria, in particular hard-to-lyse microorganisms like Streptococcus spp., Lactobacillus spp., Staphylococcus spp., Bacillus spp. and Clostridium spp..
IST Innuscreen‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. The extreme thermostability of the polymerase allows incubation at high temperatures (95 ° C). It is recommended for use in routine PCR reactions.
The innuTaq HOT-A DNA Polymerase provides improved specificity and sensitivity when amplifying low-copy-number targets in complex backgrounds or when prolonged room temperature set up is required. The polymerase activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation.
The Proteinase K is one of the most active endopeptidases known. The enzyme is extraordinarily effective against native proteins and can be used for quickly inactivating endogenous RNases and DNases. Proteinase K is particularly suitable for isolating nucleic acids for use in amplification reactions, for isolating native RNA and DNA from tissues and cell lines, for promoting cell lysis by activating a bacterial autolysis factor, and for modifying proteins and/or glycoproteins on cell surfaces (for membrane structure analyses).
