The innuPREP PME cfDNA Kit - PP Mini is based on a new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or kits. The first step is capturing of free-circulating DNA with a special polymer. Subsequently the captured free-circulating DNA is dissolved in a special lysis buffer and then the DNA is extracted.
The kit combines a unique patented technology for enrichment of biomolecules with a very efficient extraction of DNA using magnetic particles.
Starting material
Plasma, liquor, cell culture supernatant (2.5 - 3.5 ml)
Low Throughput Device
PurePrep Mini
Extract
DNA
Reactions
16 or 96 (PP Mini)
Extraction time
90 min (including all steps)
Average yield
Depending on sample quality/quantity