The innuPREP FFPE DNA Kit – PP Mini has been designed for automated isolation of genomic DNA from formalin-fixed, paraffin-embedded (FFPE) samples using the PurePrep Mini device.
The procedure starts with an external lysis step without the need for a deparaffinization step using toxic and hazardous components like octane or xylene. After the external lysis and incubation step the following extraction process runs automatically on the PurePrep Mini. The extraction process is based on binding of the DNA to surface-modified magnetic particles. After washing steps the nucleic acid is eluted from the magnetic particles with Elution Buffer and is now ready to use. The extraction procedure takes place on the magnetic particle processor PurePrep Mini and allows the parallel and flexible extraction of up 1 to 16 samples.
Starting material
FFPE tissue samples
Size: approx. 2 × 5 µm; optionally more starting material
Average yield
Depending on sample quality/quantity
Extraction time
Preliminary steps: approx. 3.5 hours
Automated Extraction protocol: approx. 30 minutes