The innuPREP Blood DNA Kit – PP Mini, prefilled has been designed for the automated isolation of genomic DNA from whole blood on the PurePrep Mini device. The extraction procedure is based on a new kind of chemistry.
The Reagent Strip of the kit is prefilled with almost all extraction reagents which are needed for the extraction process. After addition of the whole blood sample and the Proteinase K, the extraction procedure begins with sample lysis. In between the device stops and MAG Suspension F and Binding Solution SBS must be added to the lysed sample. Afterwards the Reagent Strip must be put back to the device and the automated extraction is followed by binding of DNA to surface-modified magnetic particles. After washing steps, the DNA is eluted from the magnetic particles with Elution Buffer and is now ready to use for downstream applications.
The extraction procedure takes place on the magnetic particle processor PurePrep Mini and allows the parallel and flexible extraction of 1 up to 16 samples.
The kit is intended for use by professional users. The DNA extracted using this kit is suitable for a wide range of downstream applications, such as amplification reactions and further analytical procedures.
Starting material
Whole blood (up to 200µl)
Average yield
Depending on sample quality/quantity
Extraction time
PurePrep Mini: 58 min