innuPREP Anipath DNA/RNA Kit - PP Mini, prefilled (strips)

The kit contains a Carrier Mix. The Carrier Mix consists of an essential Carrier RNA as well as a synthetic DNA fragment and MS2 RNA. Both can be used as internal extraction control, which can be detected using the innuDETECT Internal Control DNA/RNA Assay by IST Innuscreen GmbH. In addition, user-specific internal controls can be used. No data are available on the rate of recovery of user-specific internal controls. There can be no guarantee for the recovery of user-specific internal controls. It is also pointed out here that user-specific controls based on MS2 RNA sequences may lead to higher detection signals with the MS2 RNA from the Carrier Mix. It is important to note, that the kit should always be used with an internal extraction control and corresponding detection assays to monitor the purification, amplification, and detection processes. Please note that the eluates contain Carrier Mix. The detection limit for certain viruses depends on the individual procedures, for example in-house PCR or commercially used detection assays.

The kit contains 2 different extraction protocols.

Protocol 1: Lysis and Binding steps are separate

Protocol 2: Lysis and Binding steps are combined

Protocol 1 has superior sensitivity and protocol 2 is extremely fast and fully automated.

The extraction procedure takes place on the magnetic particle processor PurePrep Mini and allows the parallel and flexible extraction of 1 up to 16 samples. Additionally, the kit is compatible with the PurePrep Midi device. Please refer to the corresponding chapters of the devices.