The innuPREP PlantPath DNA/RNA Kit - KFFLX has been designed for automated isolation of viral or bacterial DNA and RNA from different kinds of starting material on the KingFisher FLEX. The extraction procedure is based on a new kind of chemistry. The kit has been successfully tested for:
Clavibacter sepedonicus (Cs)
Ralstonia solanacearum (Rs)
Viral RNA out of different kinds of potato viruses
The innuPREP Plasmid DNA Kit – PP Mini has been designed for the automated isolation of plasmid DNA from up to 5 ml of cultured bacterial cells on the PurePrep Mini device.
The innuPREP Plasmid Mini Kit 2.0 allows the fast and easy isolation of plasmid DNA from bacterial cultures. To gain an optimal result, protocol steps as well as the chemistry and spin filters were improved in comparison to the precursor product innuPREP Plasmid Mini Kit.
DW Plate, Elution Plate 200ul, Tip Comp (Consumables for PP Maxi-Kits)
DW U-well Plates & Tip Comps (Consumables for PP Midi-Kits)
Consumables for the magnetic bead-based innuPREP kits of the PurePrep Mini
The innuPREP PME cfDNA Kit - PP Mini is based on a new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or kits. The first step is capturing of free-circulating DNA with a special polymer. Subsequently the captured free-circulating DNA is dissolved in a special lysis buffer and then the DNA is extracted.
The innuPREP PME cfDNA Kit – PP Mini, prefilled has been designed for the isolation of circulating cell-free DNA (cfDNA) from 2.5 – 3.5 ml plasma, liquor & cell culture supernatant. This kit is based on new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or other commercially available kits.
Most Commercially available kits use standard nucleic acid extraction procedures based on sample lysis, binding the nucleic acids on a solid material, washing and elution of nucleic acids. Due to the very low concentration of circulating cell-free DNA in plasma and liquor, these methods require large sample volumes to obtain sufficient DNA for downstream applications. As a result, these methods are very time and work consuming, labor intensive and require a lot of reagents.
The innuPREP PME cfDNA Kit – PP Mini, prefilled has been designed for the isolation of circulating cell-free DNA (cfDNA) from 2.5 – 3.5 ml plasma, liquor & cell culture supernatant. This kit is based on new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or other commercially available kits.
Most Commercially available kits use standard nucleic acid extraction procedures based on sample lysis, binding the nucleic acids on a solid material, washing and elution of nucleic acids. Due to the very low concentration of circulating cell-free DNA in plasma and liquor, these methods require large sample volumes to obtain sufficient DNA for downstream applications. As a result, these methods are very time and work consuming, labor intensive and require a lot of reagents.
Das innuPREP PME cfDNA Kit – PP Mini, vorbefüllt wurde für die Isolierung zirkulierender zellfreier DNA (cfDNA) aus 2,5–3,5 ml Plasma, Liquor und Zellkulturüberstand entwickelt. Das Kit basiert auf einer neuen Technologie, der sogenannten PME – Polymer Mediated Enrichment (polymervermittelten Anreicherung). Im Gegensatz zu herkömmlichen Methoden und anderen kommerziell erhältlichen Kits beginnt das Verfahren nicht mit einer Probenlyse.
Die meisten kommerziell erhältlichen Kits verwenden Standardverfahren zur Nukleinsäureextraktion. Dabei wird die Probe zunächst lysiert, anschließend werden die Nukleinsäuren an ein Feststoffmaterial gebunden, gewaschen und schließlich eluiert. Aufgrund der sehr geringen Konzentration zirkulierender zellfreier DNA in Plasma und Liquor erfordern diese Methoden vergleichsweise große Probenvolumina, um ausreichend DNA für nachgelagerte Anwendungen zu gewinnen.
Dies führt dazu, dass die herkömmlichen Verfahren zeit- und arbeitsintensiv sind und einen hohen Reagenzienverbrauch aufweisen.
The innuPREP RNA Kit - IPC16, which has been optimized for the InnuPure C16 touch, makes it possible to automate RNA extraction from a wide variety of tissue samples and cells.
Each Reagent Plate is prefilled and ready to use for 8 samples.
The innuPREP RNA Kit - IPC16, which has been optimized for the InnuPure C16 touch, makes it possible to automate RNA extraction from a wide variety of tissue samples and cells.
Each Reagent Strip is prefilled and ready to use for 1 sample.
The innuPREP RNA Kit – PP Midi has been designed for automated isolation of total RNA out of different starting material.
The innuPREP RNA Kit – PP Mini has been designed for automated isolation of total RNA out of different starting material.
The innuPREP RNA Kit – PP Mini, prefilled has been designed for automated isolation of total RNA from tissue samples, eukaryotic cells, bacterial cell pellets and yeasts.
The Reagent Plate of the kit is prefilled with almost all extraction reagents which are needed for the extraction process. After addition of the lysed sample (external lysis), DNase and MAG Suspension F, the automated extraction process begins by binding of RNA to surface-modified magnetic particles. After the washing steps, the RNA is eluted from the magnetic particles with RNase-free Water and is now ready to use for downstream applications.
The innuPREP RNA Kit – PP Mini, prefilled has been designed for automated isolation of total RNA from tissue samples, eukaryotic cells, bacterial cell pellets and yeasts.
The Reagent Strip of the kit is prefilled with almost all extraction reagents which are needed for the extraction process. After addition of the lysed sample (external lysis), DNase and MAG Suspension F, the automated extraction process begins by binding of RNA to surface-modified magnetic particles. After the washing steps, the RNA is eluted from the magnetic particles with RNase-free Water and is now ready to use for downstream applications.
The innuPREP RNA Mini Kit 2.0 is the all-rounder for the extraction of total RNA. The specially optimized lysis buffer system guarantees the isolation of intact RNA and sustainably inactivates endogenous and exogenous RNases.
The innuPREP RNA Virus PLUS Kit - KFFLX can be used to automate extraction of viral RNA from up to 96 samples in just approx. 45 minutes. The method is based on magnetic particle separation and on the use of patented extraction chemistry in the KingFisher FLEX system. It can be used for isolating from a wide variety of starting materials, such as cell-free bodily fluids, biopsies, swabs and stool samples.
The innuPREP SE Blood direct HMW DNA Kit - KFFLX has been designed for automated isolation of high molecular weight DNA (HMW) from 200 µl fresh whole blood stabilized with EDTA, citrate or heparin or sampled with PAXgene® Blood DNA Tubes based on a patented technology.
The innuPREP SE Blood&Eukaryotic Cells UHMW DNA Kit - KFFLX has been designed for automated isolation of ultra high molecular weight (UHMW) from cultivated eukaryotic cells or peripheral blood mononuclear cells (PBMC) derived from fresh or frozen blood stabilized with EDTA, citrate or heparin based on a patented technology.
