The innuPREP PlantPath DNA/RNA Kit - KFFLX has been designed for automated isolation of viral or bacterial DNA and RNA from different kinds of starting material on the KingFisher FLEX. The extraction procedure is based on a new kind of chemistry. The kit has been successfully tested for:
Clavibacter sepedonicus (Cs)
Ralstonia solanacearum (Rs)
Viral RNA out of different kinds of potato viruses
The extraction procedure takes place on the magnetic particle processor KingFisher FLEX and allows the parallel extraction of up to 96 samples. The kit contains a Carrier Mix. The Carrier Mix consists of a necessary Carrier RNA as well as a synthetic DNA fragment and MS2 RNA. Both can be used as internal extraction controls. The proof can be provided by means of available assays from IST Innuscreen GmbH. In addition, individual internal controls can be used. No data are available on the rate of recovery of individual used internal controls. There can be no guarantee for the recovery of individual internal controls. Please note that the use of individual controls based on MS2 RNA sequences may lead to higher detection signals with the MS2 RNA from the Carrier Mix. Please note that the eluates contain Carrier Mix.
It is important to note, that the kit should be used with an internal extraction control and corresponding detection assays to monitor the purification, amplification, and detection processes.
Starting material
200µl potato homogenate (25 navels (potato) are homogenized in 5 mL of 50 mM phosphate buffer)
Extraction time
KingFisher FLEX lysis & extraction protocol: approx. 55minutes
Average yield
Depending on type and amount of starting material
Average purity
1,7 - 2,0