The innuPREP PME cfDNA Kit – PP Mini, prefilled has been designed for the isolation of circulating cell-free DNA (cfDNA) from 2.5 – 3.5 ml plasma, liquor & cell culture supernatant. This kit is based on new technology, called: PME – Polymer Mediated Enrichment. The procedure does not start with sample lysis, like commonly used methods or other commercially available kits.
Most Commercially available kits use standard nucleic acid extraction procedures based on sample lysis, binding the nucleic acids on a solid material, washing and elution of nucleic acids. Due to the very low concentration of circulating cell-free DNA in plasma and liquor, these methods require large sample volumes to obtain sufficient DNA for downstream applications. As a result, these methods are very time and work consuming, labor intensive and require a lot of reagents.